Plumepoppy Extract is acclimated in acceptable Chinese anesthetic for its anti-inflammatory and antibacterial activities. In this study, we advised whether M. cordata abstract and/or its above alkaloid constituents, protopine, allocryptopine, sanguinarine and chelerythrine actuate the Nrf2 signalling alleyway which regulates the announcement of cytoprotective enzymes including heme oxygenase-1 (HO-1) and thioredoxin 1. In murine macrophage RAW264.7 cells, M. cordata abstract added both mRNA and protein levels of HO-1. Of the alkaloids examined, alone sanguinarine appeared to be amenable for these effects. At the absorption of 2 μM, sanguinarine induced nuclear accession of Nrf2, added the announcement of Hmox1 gene encoding HO-1 and animated HO-1 protein levels. Sanguinarine-induced Hmox1 mRNA announcement was suppressed by SB203580, a pharmacologic inhibitor of p38 mitogen-activated protein kinases (p38 MAPKs). The upregulation of HO-1 in RAW264.7 beef by sanguinarine was, however, accompanied by abatement in corpuscle viability. Nonetheless, sanguinarine at micromolar, non-cytotoxic concentrations animated protein levels of HO-1 and thioredoxin 1 in primary cultures of animal hepatocytes. We achieve that sanguinarine may, beneath adapted conditions, access the accommodation of the enzymatic antioxidant defence arrangement via activation of the p38 MAPK/Nrf2 pathway.
