Cinobufagin is a cardiotoxic bufanolide steroid buried by the Asiatic toad Bufo gargarizans. It has agnate furnishings to digitalisand is acclimated in acceptable Chinese medicine.Cinobufagin, as able-bodied as added bufadienolides, can be abandoned from the acceptable Chinese anesthetic alleged ChanSu. ChanSu is fabricated from a aggregation of chemicals present in Bufo gargarizans secretions.
Resibufogenin can be eluted out with silica gel columnchromatography, application a 5:1 arrangement of cyclohexane to acetone for the bread-and-butter in the adaptable phase. Subsequently, cinobufagin and bufalin can be afar and antiseptic application an HPLC cavalcade with a 72:28 booze to baptize solvent. Yang et al. accepted this adjustment of abreast for cinobufagin with Proton NMR.In animal adrenocortical cells, cinobufagin inhibits the beard of aldosterone and cortisol. Cinobufagin is able to arrest the announcement of the StAR protein as able-bodied as bind the archetype agency SF-1, which commonly binds to the apostle for the StAR gene.
This after-effects in beneath StAR protein artefact and decreased levels of aldosterone and cortisol synthesis. Cinobufagin aboriginal binds to a Ca2+/K+ claret film ATPase, after inducing the phosphorylation of extracellular signal-regulated kinases(ERK). Phosphorylated ERK again blocks the SF-1 archetype agency from bounden to the apostle arena of the StAR gene.
