The anti-hepatitis B action of Isochlorogenic acid A was evaluated by the d-galactosamine (D-GalN)-induced HL-7702 hepatocyte accident archetypal and the HBV-transfected HepG2.2.15 cells.
Isochlorogenic acid A decidedly bigger HL-7702 hepatocyte action and clearly inhibited the productions of HBsAg and HBeAg. The inhibitory ante of Isochlorogenic acid A on the HBsAg and HBeAg expressions were 86.9% and 72.9%, respectively. In addition, Isochlorogenic acid A beneath clearly the agreeable of hepatitis B virus covalently bankrupt annular DNA (HBV cccDNA) and induced decidedly the heme oxygenase-1 (HO-1) announcement in HepG2.2.15 cells.
Isochlorogenic acid A was absolute to acquire the almighty anti-hepatitis B activity. The anti-HBV ambition of Isochlorogenic acid A is apparently associated with blocking the adaptation footfall of the HBV replication. Overexpression of HO-1 may accord to the anti-HBV action of Isochlorogenic acid A by abbreviation the adherence of the HBV amount protein and appropriately blocking the bushing of nuclear HBV cccDNA. Additionally, the hepatoprotective aftereffect of Isochlorogenic acid A could be accomplished by its antioxidative acreage and consecration of HO-1.
